The application follows the common liposome preparation procedure, as depicted in www.volard.wordpress.com:
(1) Stock solutions of lipids (or any other compound) are mixed in an appropriate molar ratios.
(2) Obtained mixture is purged with nitrogen and organic solvent is finally evacuated under vacuum.
(3) Obtained lipid mixture is hydrated and extruded or sonicated, according to the needs.
Application helps to fully parameterize the complex lipid mixture system, providing user with complete information regarding preparation procedure.
Concentrations (molarity and mg/ml), components moles, final solution volume etc. are calculated based upon initial data provided, such as: initial concentrations of the stock solutions, initial volumes and desired molar ratios.
Application includes customizable database for storing molecular weights of the frequently used compounds. Upon typing of solute names into component cells the software will suggest to autocomplete with stored information from the database.
Liposome Formulation can be stored and restored.
Application is fully interactive: all system parameters are updated immediately after any input from user (if it is sufficient!). For example: final molar concentration and molar ratios of the components can be adjusted at any time if molar concentrations of starting solutions and final suspension volume are provided.
Upon any of the aforementioned changes, the application will calculate the volumes of the initial solutions to fit the desired parameters.
Additional components are added by touching “(+) Solution (of)” field and deleted by touching the final “Moles (-)” field.
If molecular weights (Mw, units "g/mole") of solutes are provided as well, then concentrations in the units of grams of solute per litre of solution ("g/l" equivalent to "mg/ml") can be calculated.
This app is part of Bio, Chemistry, Engineering and Pharmacy Lab Pack, including 9 apps dealing with chemical solutions, reaction equilibrium, solubility, precipitation, acid base neutralisation reaction, titration, chemical kinetics and liposome preparation.
Preparation of initial, stock solutions may be greatly facilitated by general solution preparation apps: "Chemical Solution" and "Chemical Solution Wizard". To investigate the relative system, such as monomolecular thin surfactant film at air - water interface, please refer to "Langmuir Monolayer" app.
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